Description
Aviva Systems Biology ALB ELISA Kit (Guinea) (OKCA00286) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Guinea ALB antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ALB and incubated. The ALB found in the sample or standards competes with the biotinylated ALB for limited binding sites on the immobilized anti-Guinea ALB antibody. Excess unbound biotinylated ALB and sample or standard ALB is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ALB captured in the well and inversely proportional to the amount of ALB which was contained in the sample or standard